Multiple Objective Optimization work for optimizing aptamer binding to new targets done in collaboration with Chris Knight and Josh Knowles
CLADE: Closed Loop Aptameric Directed Evolution
Closed loop aptameric directed evolution, (CLADE) is a technique enabling simultaneous discovery, evolution, and optimization of aptamers. (Platt et al, 2009)
The technique was developed in the Kell group, and was an EPSRC-funded project.
Papers
Rowe, W., Wedge, D., Platt, M., Kell, D.B., and Knowles, J. (2010) Convergent evolution to an aptamer observed in small populations on DNA microarrays, Physical Biology 7, 036007. Online edition. Rowe, W., Wedge, D., Platt, M., Kell, D.B., and Knowles, J. (2010) Predictive Models for Population Performance on Real Biological Fitness Landscapes, Bioinformatics, 26 (17): 2145-2152. doi: 10.1093/bioinformatics/btq353 Platt, M., Rowe, W., Knowles, J., Day, P.J., Kell, D.B. (2009) Analysis of aptamer sequence-activity relationships. Integrative Biology, 1: 116-122. DOI:10.1039/b814892a. Online version Knight, C. G., Platt, M., Rowe, W., Wedge, D., Khan, F., McShea, A., Day, P.J., Knowles, J. and Kell, D.B. (2008) Array-based evolution of DNA aptamers allows modeling of an explicit sequence-fitness landscape. Nucleic Acids Research 37 (1): e6. doi: 10.1093/nar/gkn899. Nucleic Acids Advance Acess Data
Analysis of aptamer binding sequence-activity relationships using microarrays Analysis of the Complete Sequence-Fitness Landscape of a DNA Aptamer [ Back ] to Research Main Page